人组蛋白H3（核内参）即用型免疫组化试剂盒-分析方法-资讯-生物在线

人组蛋白H3（核内参）即用型免疫组化试剂盒

英文名称

Human Histone H3 Ready-To-Use IHC Kit

中文名称

人组蛋白H3（核内参）即用型免疫组化试剂盒

别名

H3 histone family member E pseudogene; H3 histone family, member A; H3/A; H31_HUMAN; H3F3; H3FA; Hist1h3a; HIST1H3B; HIST1H3C; HIST1H3D; HIST1H3E; HIST1H3F; HIST1H3G; HIST1H3H; HIST1H3I; HIST1H3J; HIST3H3; histone 1, H3a; Histone cluster 1, H3a; Histone H3 3 pseudogene; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l; H3.1; H3/d; H3C1; H3C10; H3C11; H3C12; H3C2; H3C3; H3C4; H3C7; H3C8; H3FD

种属

human

应用范围

Immunohistochemistry

适用样品基质

PFPE tissue

描述

IHC0102 is a ready-to-use IHC kit for staining of Histone H3. The kit provides all reagents, from antigen retrieval to cover slip mounting, that require little to no diluting or handling prior to use. Simply apply the reagents to your sample slide according to the protocol and you're steps away from obtaining high-quality IHC data.

保存条件

Please store components at the temperatures indicated on the individual tube labels. The kit is stable for 6 months from the date of receipt.

注意事项

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

产品介绍

产品组分及规格

编号	组分	规格	浓度	储存
1	PBS缓冲液（干粉）	2 L×2	20x	室温
2	抗原修复缓冲液	20 ml	100x	2-8℃
3	内源性过氧化物酶阻断剂	3 ml	RTU	2-8℃
4	封闭工作液	3 ml	RTU	2-8℃
5	一抗（Human Histone H3 Rabbit pAb）	6 ml	RTU	2-8℃
6	二抗（HRP-Goat anti-Rabbit IgG pAb）	6 ml	RTU	2-8℃
7	DAB kit （20×）显色液	0.3 ml	RTU	-20℃
8	DAB kit （20×）稀释液	0.3 ml	RTU	-20℃
9	复染试剂	5 ml	RTU	室温
10	封片剂	5 ml	RTU	室温
11	对照切片（人肾）	1张	RTU	室温
12	说明书	1份

背景

Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3 along with four core histone proteins binds to DNA forming the structure of the nucleosome. Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Post translationally, histones are modified in a variety of ways to either directly change the chromatin structure or allow for the binding of specific transcription factors. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of post-translational modification that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

石蜡包埋组织的免疫组织化学方案

1. 脱蜡水化

石蜡切片置于新鲜二甲苯中浸泡脱蜡3次，每次15 min；依次置于不同浓度（100%、95%、90%、80%、70%）乙醇浸泡各5 min，再置于蒸馏水洗涤5 min，重复3次。

2. 抗原修复

沸水浴修复：将100×抗原修复缓冲液（试剂2）用蒸馏水稀释成1×抗原修复缓冲液，放入修复盒中并提前加热至95-100℃（注意盖好以防液体蒸发），然后将切片放入修复盒中，在沸水浴环境中保持外沸状态15 min，室温自然冷却；用PBS缓冲液（试剂1，将干粉溶解在2L蒸馏水中）清洗5 min，重复3次。

3. 阻断内源性过氧化物酶

用吸水纸吸去玻片上多余的液体，用免疫组化笔在组织周围画圈，加入2-4滴内源性过氧化物酶阻断剂（试剂3），室温下置于湿盒中孵育15 min，用PBS洗涤5 min，重复3次。

4. 血清封闭

用吸水纸吸去玻片上多余的液体，加入2-4滴封闭工作液（试剂4）

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