PRKCZ monoclonal antibody (M01), clone 2D1
产品名称： PRKCZ monoclonal antibody (M01), clone 2D1
英文名称： PRKCZ monoclonal antibody (M01), clone 2D1
产品编号： H00005590-M01
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Mouse monoclonal antibody raised against a partial recombinant PRKCZ.
- Immunogen:
- PRKCZ (AAH08058, 165 a.a. ~ 255 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
- Sequence:
- KLLVHKRCHGLVPLTCRKHMDSVMPSQEPPVDDKNEDADLPSEETDGIAYISSSRKHDSIKDDSEDLKPVIDGMDGIKISQGLGLQDFDLI
- Host:
- Mouse
- Reactivity:
- Human
- Isotype:
- IgG2b Kappa
- Storage Buffer:
- In 1x PBS, pH 7.2
- Storage Instruction:
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
- Quality Control Testing:
- Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (35.42 KDa) .
- MSDS:
Download
- Applications
- Western Blot (Transfected lysate)
- Western Blot analysis of PRKCZ expression in transfected 293T cell line by PRKCZ monoclonal antibody (M01), clone 2D1.
Lane 1: PRKCZ transfected lysate(67.7 KDa).
Lane 2: Non-transfected lysate. - Protocol Download
- Western Blot (Recombinant protein)
- Protocol Download
- Sandwich ELISA (Recombinant protein)
- Detection limit for recombinant GST tagged PRKCZ is approximately 0.1ng/ml as a capture antibody.
- Protocol Download
- RNAi Knockdown (Antibody validated)
- Western blot analysis of PRKCZ over-expressed 293 cell line, cotransfected with PRKCZ Validated Chimera RNAi ( Cat # H00005590-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with PRKCZ monoclonal antibody (M01) clone 2D1 (Cat # H00005590-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Protocol Download
- Entrez GeneID:
- 5590
- GeneBank Accession#:
- BC008058
- Protein Accession#:
- AAH08058
- Gene Name:
- PRKCZ
- Gene Alias:
- PKC-ZETA,PKC2
- Gene Description:
- protein kinase C, zeta
- Omim ID:
- 176982
- Gene Ontology:
- Hyperlink
- Gene Summary:
- Protein kinase C (PKC) zeta is a member of the PKC family of serine/threonine kinases which are involved in a variety of cellular processes such as proliferation, differentiation and secretion. Unlike the classical PKC isoenzymes which are calcium-dependent, PKC zeta exhibits a kinase activity which is independent of calcium and diacylglycerol but not of phosphatidylserine. Furthermore, it is insensitive to typical PKC inhibitors and cannot be activated by phorbol ester. Unlike the classical PKC isoenzymes, it has only a single zinc finger module. These structural and biochemical properties indicate that the zeta subspecies is related to, but distinct from other isoenzymes of PKC. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq
- Other Designations:
- OTTHUMP00000001368,OTTHUMP00000044160
- Interactome
