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产品名称：神经酰胺激酶CERK抗体说明书
产品货号：YM-H17185-X
浓 度： 1mg/1ml
贮 存： 贮存于-20℃.
亚 型： IgG
性 状：Lyophilized or Liquid
产品类型： 一抗 多抗体
纯化方法 affinity purified by Protein A
储 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
神经酰胺激酶CERK抗体说明书产品WB实验中的对照系统：
he chromogenic reaction （Note: two anti generally have two kinds ofcommonly used marker enzyme, HRP and AP, chromogenic substrate kit andits corresponding to DAB and BCIP/NBT, the general use ofchemiluminescence lysate.） Westernbloting is the first to transfer afterelectrophoresis separation of proteins from the gel onto a NC membrane,there are generally two ways: capillary blotting and electrophoreticblotting. Westernbloting is a common method used to detect the protein,through the immune reaction of antigen antibody can be quantitativelymeasured the relative molecular weight of antibody, and the relativeabundance of known antigen or antibody and the relation between. Atpresent, it is applied in many fields, detection of infectious diseasesand autoimmune diseases, allergies, etc. in clinic, in antibodyproduction field it with ELISA, ICC, IHC, IFA and other immunetechnology has become the main means of engaging a screening ofmonoclonal antibody production.
WB对照系统
阳性对照：最好有标准品，或阳性血清、阳性上清。
阴性对照：测血时用相应小鼠未免疫血清，测培养上清，用无克隆培养上清。
空白对照：不加一抗，用1%BSA代替。
无关对照（替代对照）：用无关项目一抗，或无关项目的抗体。
The principle of immunohistochemical method:
1. Immunofluorescence cytochemistry technology
2. Immune enzyme cytochemistry technology: within the cell or tissue on the corresponding antigen to locate or qualitative research.
3. Immune colloidal gold technique: with colloidal gold mark is A resistance, the combination of the two can resist or other specific immunoglobulin molecules (such as staphylococcus protein A) as A probe to qualitative tissue or cell antigen, positioning and quantitative research.
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