Calreticulin,钙网蛋白抗体

产品编号HZ-5913R
英文名称Calreticulin
中文名称钙网蛋白抗体
别 名Autoantigen RO; CALR; CALR protein; CALR_HUMAN; Calregulin; Calreticulin; cC1qR; CRP55; CRTC; Endoplasmic reticulum resident protein 60; ERp60; grp60; HACBP; RO; Sicca syndrome antigen A; SSA.
说 明 书0.1ml 0.2ml
研究领域细胞生物 免疫学 信号转导 细胞表面分子
抗体来源Rabbit
克隆类型Polyclonal
交叉反应Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, Guinea Pig,
Calreticulin,钙网蛋白抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1:50-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量44kDa
细胞定位细胞浆 细胞膜 细胞外基质 分泌型蛋白
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human Calreticulin
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Calreticulin,钙网蛋白抗体PubMedPubMed
产品介绍background:
Molecular calcium-binding chaperone promoting folding, oligomeric assembly and quality control in the ER via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export.

Subunit:
Monomer. Component of an EIF2 complex at least composed of CELF1/CUGBP1, CALR, CALR3, EIF2S1, EIF2S2, HSP90B1 and HSPA5. Interacts with PDIA3/ERp57 (By similarity). Interacts with NR3C1 and TRIM21. Interacts with GABARAP.

Subcellular Location:
Endoplasmic reticulum lumen. Cytoplasm, cytosol. Secreted, extracellular space, extracellular matrix. Cell surface.

Similarity:
Belongs to the calreticulin family.

Gene ID:
811

Calreticulin,钙网蛋白抗体Database links:
Entrez Gene: 41166 Fruit fly (Drosophila melanogaster)
Entrez Gene: 811 Human
Entrez Gene: 12317 Mouse
Entrez Gene: 100009050 Rabbit
Entrez Gene: 64202 Rat
Omim: 109091 Human
SwissProt: P29413 Fruit fly (Drosophila melanogaster)
SwissProt: P27797 Human
SwissProt: P14211 Mouse
SwissProt: P15253 Rabbit
SwissProt: P18418 Rat
Unigene: 2457 Fruit fly (Drosophila melanogaster)
Unigene: 515162 Human
Unigene: 1971 Mouse
Unigene: 467043 Mouse
Unigene: 974 Rat

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品图片

Tissue/cell: human placenta tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-Calreticulin Polyclonal Antibody, Unconjugated(HZ-5913R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining