SRC (N-term) Peptide
产品名称： SRC (N-term) Peptide
英文名称： SRC (N-term) Peptide
产品编号： P2761
产品价格： 0
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围： null
亚诺法生技股份有限公司（Abnova)
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- 邮编 : 11493
- 所在区域 : 台湾
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- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Mouse monoclonal antibody raised against full length recombinant SRC.
- Immunogen:
- Recombinant protein corresponding to full length human SRC.
- Host:
- Mouse
- Reactivity:
- Human, Mouse
- Form:
- Liquid
- Purification:
- Protein G purification
- Isotype:
- IgG1
- Storage Buffer:
- In PBS (0.09% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- ELISA (1:1000)
Western Blot (1:100-500)
Immunohistochemistry (1:50-100)
Immunoprecipitation (2 ug/test)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- Microexon-based regulation of ITSN1 and Src SH3 domains specificity relies on introduction of charged amino acids into the interaction interface.
Dergai M, Tsyba L, Dergai O, Zlatskii I, Skrypkina I, Kovalenko V, Rynditch A.Biochem Biophys Res Commun. 2010 Aug 20;399(2):307-12. Epub 2010 Jul 24.
- 2.
- Expression of a Src family kinase in chronic myelogenous leukemia cells induces resistance to imatinib in a kinase-dependent manner.
Pene-Dumitrescu T, Smithgall TE.J Biol Chem. 2010 Jul 9;285(28):21446-57. Epub 2010 May 7.
- 3.
- Human phosphatidylethanolamine-binding protein 4 promotes transactivation of estrogen receptor alpha (ERalpha) in human cancer cells by inhibiting proteasome-dependent ERalpha degradation via association with Src.
Liu H, Qiu J, Li N, Chen T, Cao X.J Biol Chem. 2010 Jul 16;285(29):21934-42. Epub 2010 May 11.
- Applications
- Western Blot (Cell lysate)
- The SRC monoclonal antibody, clone 17AT28 (Cat # MAB1155) is used in Western blot to detect SRC in Jurkat cell lysate.
- Western Blot (Transfected lysate)
- FG Pancreatic Carcinoma Cell Lines stably expressing vector along (FG-V) the beta3 integrin subunit (FG-beta3) or a beta3 truncation mutant (FG-759x) . SRC monoclonal antibody, clone 17AT28 (Cat # MAB1155) was diluted 1 : 500 in 1% BSA/TBST and incubated overnight at 4 °C. After washing 3 x 5 min with TBST the blots were incubated with 1 : 5000 goat anti-mouse or goat anti-rabbit secondary antibody for 1 hr at room temperature. The blots were again washed 3 x 5 min with TBST and developed using ECL reagent. Data is kindly provided by Dr. Weis of Cheresh Lab, UCSD.
- Entrez GeneID:
- 6714
- Protein Accession#:
- P12931
- Gene Name:
- SRC
- Gene Alias:
- ASV,SRC1,c-SRC,p60-Src
- Gene Description:
- v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian)
- Omim ID:
- 190090
- Gene Ontology:
- Hyperlink
- Gene Summary:
- This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq
- Other Designations:
- OTTHUMP00000174476,OTTHUMP00000174477,proto-oncogene tyrosine-protein kinase SRC,protooncogene SRC, Rous sarcoma,tyrosine kinase pp60c-src,tyrosine-protein kinase SRC-1
- Related Disease