Recombinant Rat TNF-α

Catalog Number:

400-14

Synonyms:

Tumor Necrosis Factor, TNFSF2, Cachectin, Differentiation-inducing factor (DIF), Necrosin, Cytotoxin

Description:

TNF-α is a pleiotropic pro-inflammatory cytokine secreted by various cells including adipocytes, activated monocytes, macrophages, B cells, T cells and fibroblasts. It belongs to the TNF family of ligands and signals through two receptors, TNFR1 and TNFR2. TNF-α is cytotoxic to a wide variety of tumor cells and is an essential factor in mediating the immune response against bacterial infections. TNF-α also plays a role in the induction of septic shock, auto immune diseases, rheumatoid arthritis, inflammation, and diabetes. Recombinant rat TNF-α is a soluble 157 amino acid protein (17.3 kDa) which corresponds to C-terminal extracellular domain of the full length transmembrane protein.

AA Sequence:

MLRSSSQNSS DKPVAHVVAN HQAEEQLEWL SQRANALLAN GMDLKDNQLV VPADGLYLIY SQVLFKGQGC PDYVLLTHTV SRFAISYQEK VSLLSAIKSP CPKDTPEGAE LKPWYEPMYL GGVFQLEKGD LLSAEVNLPK YLDITESGQV YFGVIAL

Source:

E.coli

Purity:

Greater than 98% by SDS-PAGE gel and HPLC analyses.

Biological Activity:

The ED₅₀ as determined by the cytolysis of murine L929 cells in the presence of actinomycin D is ≤ 0.05 ng/ml, corresponding to a specific activity of ≥ 2 x 10⁷ units/mg.

Cross Reactivity:

Mouse, Rat, Bacteria, Mouse + Chicken, Human, Monkey

Research Interests:

	AIDS / HIV		Angiogenesis/ Cardiovascular
	Apoptosis		Cancer
	Diabetes / Weight Regulation		Immune System
	Inflammation		Neurobiology
	Stem Cells & Differentiation		TNF Superfamily
	Allergy		Transplantation