HILOAD 16/10 Q SEPHAROSE HP-色谱介质-耗材-生物在线
上海基星生物科技有限公司
HILOAD 16/10 Q SEPHAROSE HP

HILOAD 16/10 Q SEPHAROSE HP

商家询价

产品名称： HILOAD 16/10 Q SEPHAROSE HP

英文名称： HILOAD 16/10 Q SEPHAROSE HP

产品编号： 17-1064-01

产品价格： 0

产品产地： 瑞典

品牌商标： 美国GE

更新时间： null

使用范围： null

上海基星生物科技有限公司
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进入展台

17-1064-01 HILOAD 16/10 Q SEPHAROSE HP
17-1066-01 HILOAD 26/10 Q SEPHAROSE HP
17-1068-01 HILOAD 16/60 SUPERDEX 75 PG
17-1069-01 HILOAD 16/60 SUPERDEX 200 PG
17-1070-01 HILOAD 26/60 SUPERDEX 75 PG
17-1071-01 HILOAD 26/60 SUPERDEX 200 PG
17-1085-01 HILOAD 16/10 PHENYL SEPHAROSE
17-1086-01 HILOAD 26/10 PHENYL SEPHAROSE
17-1137-01 HILOAD 16/10 SP SEPHAROSE HP
17-1138-01 HILOAD 26/10 SP SEPHAROSE HP
17-1139-01 HILOAD 16/60 SUPERDEX 30 PG
17-1140-01 HILOAD 26/60 SUPERDEX 30 PG

HiLoad™ IEX HP Columns and IEX Sepharose™ High Performance Lab Packs

  • High-performance preparative separations where high efficiency and high resolution are desired.
  • Highly cross-linked agarose matrix provides excellent chemical and physical stability.
  • Available as strong anion exchangers (Q Sepharose HP) and strong cation exchangers (SP Sepharose HP), analogous to Mono Q™ and S, and Q and SP Sepharose™ Fast Flow.
  • Easy to use. For optimal performance and reproducibility, Sepharose™ High Performance is also available in prepacked HiTrap™ columns.

HiLoad™ IEX HP Columns and IEX Sepharose™ High Performance Lab Packs

Technical Information


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Sepharose™ High Performance ion exchangers provide highly efficient, high-resolution preparative scale separations.

 

TECHNICAL SPECIFICATIONS
Ion exchanger type   
Q Sepharose™ HP  Quaternary ammonium strong anion exchanger 
SP Sepharose™ HP  Sulfopropyl strong cation exchanger 
Charged groups   
  SP Sepharose™ HP  - SO3- 
  Q Sepharose™ HP  - N+(CH3)3 
Ionic capacity   
Q Sepharose™ HP  0.14-0.20 mmol Cl-/ml 
SP Sepharose™ HP  0.15-0.20 mmol H+/ml 
Dynamic capacity   
Q Sepharose™ HP  70 mg BSA/ml medium 
SP Sepharose™ HP  55 mg ribonuclease/ml medium 
Rec. flow velocity  30-150 cm/h 
Max. pressure over the packed bed in a HiLoad column during operation  3 bar (42 psi, 0.3 MPa) 
HiLoad™ column hardware pressure limit  5 bar (73 psi, 0.5 MPa) 
Average particle size  34 μm 
Exclusion limit (Mr)  » 4 ×106 globular protein 
Matrix  cross-linked agarose, 6% 
pH stability   
Q Sepharose™ HP  2-12 (working and long term),
1-14 (short term) 
SP Sepharose™ HP  4-13 (working and long term),
3-14 (short term) 
Chemical stability  Stable in all commonly used buffers:  8 M urea, 6 M guanidine HCl, 70% ethanol, 1 M NaOH,* 1 M acetic acid* 
Storage  20% ethanol (Q), 0.2 M sodium acetate in 20% ethanol (SP) 
Storage temperature  4°C to 30°C 
Prepacked HiLoad™ Columns
Bed volume   
HiLoad™ 16/10  20 ml  
HiLoad™ 26/10  53 ml 
Bed dimensions   
HiLoad™ 16/10  16 mm × 100 mm 
HiLoad™ 26/10  26 mm × 100 mm 
Efficiency  > 12 000 m-1